Gene Silencing Induced by Cytosine Methylation in Transgenic Tomato
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چکیده
منابع مشابه
Tomato and Tobacco Phytoene Desaturase Gene Silencing by Virus-Induced Gene Silencing (VIGS) Technique
Background and Aims: Virus-Induced Gene Silencing (VIGS) is a virus vector technology that exploits antiviral defense mechanism. By infecting plants with recombinant viruses containing host genes inserted in the viral genome, VIGS achieves the RNA silencing process. The purpose of this study was to investigate the possibility of tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana be...
متن کاملtomato and tobacco phytoene desaturase gene silencing by virus-induced gene silencing (vigs) technique
background and aims: virus-induced gene silencing (vigs) is a virus vector technology that exploits antiviral defense mechanism. by infecting plants with recombinant viruses containing host genes inserted in the viral genome, vigs achieves the rna silencing process. the purpose of this study was to investigate the possibility of tomato (lycopersicon esculentum mill.) and tobacco (nicotiana bent...
متن کاملCytosine methylation associated with repeat-induced point mutation causes epigenetic gene silencing in Neurospora crassa.
Repeated DNA sequences are frequently mutated during the sexual cycle in Neurospora crassa by a process named repeat-induced point mutation (RIP). RIP is often associated with methylation of cytosine residues in and around the mutated sequences. Here we demonstrate that this methylation can silence a gene located in nearby, unique sequences. A large proportion of strains that had undergone RIP ...
متن کاملEffectively organ-specific virus induced gene silencing in tomato plant
Virus-induced gene silencing (VIGS) is a valuable tool for identification and characterization of genes function. To improve the efficiency of VIGS in different organ, we developed an organ-specific VIGS that could be applied to tomato (Solanum lycopersicum cv Micro Tom) leaves, flowers and fruits respectively. With phytoene desaturase (PDS) as a reporter gene, almost up to 100% of efficiency o...
متن کاملVirus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
RNA interference (RNAi) is a highly specific gene-silencing phenomenon triggered by dsRNA. This silencing mechanism uses two major classes of RNA regulators: microRNAs, which are produced from non-protein coding genes and short interfering RNAs (siRNAs). Plants use RNAi to control transposons and to exert tight control over developmental processes such as flower organ formation and leaf develop...
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ژورنال
عنوان ژورنال: Journal of Plant Biotechnology
سال: 2007
ISSN: 1229-2818
DOI: 10.5010/jpb.2007.34.4.323